Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization

作者：Kiboku Takayuki; Katoh Tsuyoshi; Nakamura Akio; Kitamura Akira; Kinjo Masataka; Murakami Yota; Takahashi Masayuki^*

来源：Genes to Cells, 2013, 18(2): 90-109.

DOI：10.1111/gtc.12021

Nonmuscle myosin II forms a folded conformation (10S form) in the inactivated state; however, the physiological importance of the 10S form is still unclear. To investigate the role of 10S form, we generated a chimeric mutant of nonmuscle myosin IIB (IIB-SK1 center dot 2), in which S1462-R1490 and L1551-E1577 were replaced with the corresponding portions of skeletal muscle myosin heavy chain. The IIB-SK1 center dot 2 mutant did not fold into a 10S form under physiological condition in vitro. IIB-SK1 center dot 2 was less dynamic by stabilizing the filamentous form and accumulated in the posterior region of migrating cells. IIB-SK1 center dot 2 functioned properly in cytokinesis but altered migratory properties; the rate and directional persistence were increased by IIB-SK1 center dot 2 expression. Surprisingly, endogenous nonmuscle myosin IIA was excluded from the posterior region of migrating cells expressing IIB-SK1 center dot 2, which may underlie the change of the cellular migratory properties. These results suggest that the 10S form is necessary for maintaining nonmuscle myosin II in an unassembled state and for recruitment of nonmuscle myosin II to a specific region of the cell.

• 出版日期2013-2