A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

作者：Lorena Alvarado Gamez Ana; Asuncion Alonso Lomillo Maria; Dominguez Renedo Olga; Julia Arcos Martinez Maria

来源：Sensors (Switzerland), 2014, 14(2): 3756-3767.

DOI：10.3390/s140203756

• alkaline phosphatase
• biosensor
• gold nanoparticles
• 4-nitrophenyl phosphate
• screen printed electrode
• vanadium
• water analysis

A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 +/- 0.06 M, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.

• 出版日期2014-2