Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function

作者：Ko Jae hyeong; Wang Yane Shih; Nakamura Akiyoshi; Guo Li Tao; Soell Dieter; Umehara Takuya

来源：FEBS Letters, 2013, 587(19): 3243-3248.

DOI：10.1016/j.febslet.2013.08.018

• Pyrrolysyl-tRNA synthetase
• Non-canonical amino acid
• Phenylalanyl-tRNA synthetase
• Superfolder green fluorescent protein

Pyrrolysyl-tRNA synthetase (PylRS) is a class IIc aminoacyl-tRNA synthetase that is related to phenylalanyl-tRNA synthetase (PheRS). Genetic selection provided PylRS variants with a broad range of specificity for diverse non-canonical amino acids (ncAAs). One variant is a specific phenylalanine-incorporating enzyme. Structural models of the PylRSamino acid complex show that the small pocket size and pi-interaction play an important role in specific recognition of Phe and the engineered PylRS active site resembles that of Escherichia coli PheRS.

• 出版日期2013-10-1