Assay of hydroxyl radicals generated by focused ultrasound

作者：Villeneuve L^*; Alberti L; Steghens J P; Lancelin J M; Mestas J L

来源：Ultrasonics Sonochemistry, 2009, 16(3): 339-344.

DOI：10.1016/j.ultsonch.2008.09.007

• Cavitation
• Ultrasound
• Sonolysis
• Terephthalic acid
• Hydroxyl radicals
• Fluorescence

Water sonolysis leads to the formation of hydroxyl radicals (OK). Various techniques are used to detect the OH(center dot) production and thus to assess the level of ultrasound-mediated cavitation generated in vitro. In this study, we used terephthalic acid (TA) as an OH(center dot) trap. This method is based on the fluorescent properties of hydroxyterephthalic acid (HTA) formed by the reaction of TA with OH(center dot) and used as an indicator of the degree of inertial cavitation caused.
The experimental system is comprised mainly of a focused piezoelectric ultrasound transmitter and a measurement cell containing 1 X PBS/TA diluted solution. In the first part, we aimed to characterize the most appropriate experimental conditions (TA dosimeter solution, irradiation time) in order to optimize the resulting HTA fluorescence values. Then, we could determine that the HTA production increased with the level of the cavitation phenomenon caused by the acoustic power from which OR production may be estimated.

• 出版日期2009-3