A Gain-of-Function Mutation in Gating of Corynebacterium glutamicum NCgl1221 Causes Constitutive Glutamate Secretion

作者：Nakayama Yoshitaka^*; Yoshimura Kenjiro; Iida Hidetoshi

来源：Applied and Environmental Microbiology, 2012, 78(15): 5432-5434.

DOI：10.1128/AEM.01310-12

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.

• 出版日期2012-8