A Method for the Simultaneous Determination of 3T3-L1 Adipocyte Metabolites by Liquid Chromatography/Mass Spectrometry Using [C-13]-stable Isotopes

作者：Nagai Hiroyuki^*; Goto Tsuyoshi; Takahashi Nobuyuki; Kusudoh Tatsuya; Deyashiki Yoshihiro; Esaka Yukihiro; Kawada Teruo

来源：Bioscience Biotechnology and Biochemistry, 2011, 75(8): 1485-1489.

DOI：10.1271/bbb.110192

• peroxisome proliferator-activated receptor-gamma (PPAR gamma)
• liquid chromatography mass spectrometry (LC/MS)
• de novo fatty acid synthesis
• thiazolidinedione (TZD)
• screening system

A useful method employing liquid chromatography mass spectrometry (LC/MS) and a stable isotope was developed for simultaneous examination of major metabolism in adipocytes, de novo fatty acid synthesis, glycerol output, and glucose uptake with high sensitivity. The addition of thiazolidinediones, potent agonists of peroxisome proliferator-activated receptors-gamma, for 10 d increased glucose uptake in a dose-dependent manner. Fatty acid (FA) synthesis increased at low concentrations of thiazolidinediones (TZDs) and decreased at high concentrations. It is important to assess adipocytes from various examples of metabolism, because each example of adipocyte metabolism is directly related to obesity or metabolic syndrome in various ways. The technique makes metabolic examination easier than conventional methods by means of radioisotopes and makes it possible to identify metabolites and to apply them in biomarker screening.

• 出版日期2011-8