Antisense Ki-67 cDNA transfection reverses the tumorigenicity and induces apoptosis in human breast cancer cells

作者：Wang, Rui; Luo, Danfeng; Ma, Xiangyi; Yang, Wanhua; Chen, Rui; Liu, Yan; Meng, Li; Zhou, Jianfeng; Xu, Gang; Lu, Yun-Ping; Wang, Shixuan; Ma, Ding^*

来源：Cancer Investigation, 2008, 26(8): 830-835.

DOI：10.1080/07357900801941878

• Ki-67
• breast cancer
• antisense
• proliferation
• invasion

Effects of antisense Ki-67 cDNA transfection on breast cancer cells were investigated in this study. Transfection of antisense Ki-67 cDNA resulted in a 70%-80% reduction in proliferation of MDA-MB-435s, cells which highly expressed Ki-67 mRNA and protein. Transwell assay showed that mobility and invasion capability was dramatically inhibited by 50%-60%, and cell cycle analysis displayed a higher proportion in G2/M and G0/G1 phases accompanied by remarkably increased ratio of apoptotic cells. Our results suggested that antisense Ki-67 cDNA vector treatment might be an important potential option in the anticancer therapy.

• 出版日期2008
• 单位华中科技大学