An archaeal NADH oxidase causes damage to both proteins and nucleic acids under oxidative stress

作者：Jia Baolei; Lee Sangmin; Pham Bang P; Cho Yoon Seung; Yang Jae Kyung; Byeon Hee Seop; Kim Jong Cheol; Cheong Gang Won^*

来源：Molecules and Cells, 2010, 29(4): 363-371.

DOI：10.1007/s10059-010-0045-8

• cell death
• dual function
• NADH oxidase
• protein aggregation
• thermophilic archaeon

NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to H(2)O(2) or four-electron reduction of oxygen to H(2)O. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to beta-amyloid and Lewy bodies in neurodegenerative diseases, including an increase of beta-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce H(2)O(2). Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treatment with H(2)O(2). As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.

• 出版日期2010-4