Analysis of risk factors and prevalence of haemoplasma infection in dogs

作者：Aquino L C^*; Kamani J; Haruna A M; Paludo G R; Hicks C A; Helps C R; Tasker S

来源：Veterinary Parasitology, 2016, 221: 111-117.

DOI：10.1016/j.vetpar.2016.03.014

• Haemoplasmas
• Nigeria
• Dogs
• Prevalence
• Risk factors

Mycoplasma haemocanis (Mhc) and 'Candidates Mycoplasma haematoparvum' (CMhp) are canine haemoplasma species that can induce anaemia in immunocompromised and/or splenectomised dogs. This study aimed to determine the prevalence and phylogeny of canine haemoplasma species in dogs from Nigeria and describe any risk factors for infection. Canine haemoplasma species-specific and generic haemoplasma qPCR assays were used. The species-specific qPCR assays found Mhc infection in 18 of 245 dogs (7.3%), and CMhp infection in only one dog (0.4%). The generic haemoplasma OCR assays were positive in 44 of 245 (17.9%) dogs. Twenty-five dogs had discordant qPCR results in that they were generic haemoplasma qPCR positive but species-specific qPCR negative. Further evaluation of these dogs by 16S rDNA sequencing gave limited results but 5 were confirmed to be infected with non-haemoplasma species: 2 Anaplasma phagocytophilum, 1 Anaplasma ovis, 1 Serratia marcescens and 1 Aerococcus spp. The 16S rRNA gene sequences from Mhc species showed >99.8% identity with each other and >99.6% identity with GenBank sequences, and resided in a single Glade with other global Mhc and Mycoplasma haemofelis sequences, indicating low 16S rRNA genetic variability amongst this canine haemoplasma species.

• 出版日期2016-5-15