An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis

作者：Higa Yumiko; Uemura Ryoko^*; Yamazaki Wataru; Goto Shinya; Goto Yoshitaka; Sueyoshi Masuo

来源：Journal of Veterinary Medical Science, 2016, 78(8): 1343-1346.

DOI：10.1292/jvms.15-0459

• cattle
• loop-mediated isothermal amplification (LAMP) assay
• Mycoplasma bovis
• respiratory disease

We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M bovis mycoplasmas and eight non-mycoplasma bacterial strains. M bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.

• 出版日期2016-8