Use of on-line supercritical fluid extraction-supercritical fluid chromatography/tandem mass spectrometry to analyze disease biomarkers in dried serum spots compared with serum analysis using liquid chromatography/tandem mass spectrometry

作者：Suzuki Makoto; Nishiumi Shin; Kobayashi Takashi; Sakai Arata; Iwata Yosuke; Uchikata Takato; Izumi Yoshihiro; Azuma Takeshi; Bamba Takeshi; Yoshida Masaru^*

来源：Rapid Communications in Mass Spectrometry, 2017, 31(10): 886-894.

DOI：10.1002/rcm.7857

RationaleThe analytical stability and throughput of biomarker assays based on dried serum spots (DSS) are strongly dependent on the extraction process and determination method. In the present study, an on-line system based on supercritical fluid extraction-supercritical fluid chromatography coupled with tandem mass spectrometry (SFE-SFC/MS/MS) was established for analyzing the levels of disease biomarkers in DSS.
MethodsThe chromatographic conditions were investigated using the ODS-EP, diol, and SIL-100A columns. Then, we optimized the SFE-SFC/MS/MS method using the diol column, focusing on candidate biomarkers of oral, colorectal, and pancreatic cancer that were identified using liquid chromatography (LC)/MS/MS.
ResultsBy using this system, four hydrophilic metabolites and 17 hydrophobic metabolites were simultaneously detected within 15min. In an experiment involving clinical samples, PC 16:0-18:2/16:1-18:1 exhibited 93.8% sensitivity and 64.3% specificity, whereas PC 17:1-18:1/17:0-18:2 showed 81.3% sensitivity and 92.9% specificity for detecting oral cancer. In addition, assessments of the creatine levels demonstrated 92.3% sensitivity and 78.6% specificity for detecting colorectal cancer.
ConclusionsThe results of this study indicate that our method has great potential for clinical diagnosis and would be suitable for large-scale screening.

• 出版日期2017-5-30