Amelioration of carbon tetrachloride induced oxidative stress in kidney and lung tissues by ethanolic rhizome extract of Podophyllum hexandrum in Wistar rats

作者：Ganie Showkat Ahmad; Haq Ehtishamul; Masood Akbar; Zargar Mohmmad Afzal^*

来源：Journal of Medicinal Plants Research, 2010, 4(16): 1673-1677.

• Antioxidants
• carbon tetrachloride
• Podophyllum hexandrum
• GSH
• catalase

Antioxidant activity of 70% ethanolic extract of rhizome of Podophyllum hexandrum (Indian Mayapple) was investigated in rats by inducing kidney and lung injury with CCl(4). 70% ethanolic extract exhibited significant antioxidant activity in the tissues by showing increased levels of proteins, reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione-S-transferase (GST) as well as decreased levels of thiobarbituric acid reactive substances (TBARS). The 70% ethanolic extract at a dose level of 20, 30 and 50 mg/kg-day, showed a dose dependent restorative activity against CCl(4)-induced kidney and lung tissue damage. In our previous study 70% ethanolic extract of P. hexandrum had displayed a potent free radical scavenging activity under in vitro conditions. In the present study, the 70% ethanolic extract of P. hexandrum was effective in restoring CCl(4)-intoxicated kidney and lung tissue enzymes towards normalcy in combating oxidative stress in vivo. Thus, the extract has antioxidative activity.

• 出版日期2010-8-18