Design of a 5 ' exonuclease-based real-time PCR assay for simultaneous detection of Bacillus licheniformis, members of the 'B-cereus group' and B-fumarioli in gelatine

作者：De Clerck E^*; Van Mol K; Jannes G; Rossau R; De Vos P

来源：Letters in Applied Microbiology, 2004, 39(1): 109-115.

DOI：10.1111/j.1472-765X.2004.01550.x

• gelatinase
• identification
• public health
• SmartCycler
• Taqman

Aims: The design of a fast, sensitive and specific detection method for Bacillus licheniformis, members of the 'B. cereus group' and B. fumarioli in gelatine.
Methods and Results: Specific Taqman probes were designed and tested in a real-time PCR setting. A specific fluorescent signal could be obtained for all gelatine isolates attributed to these species in one single real-time PCR reaction. After sample preparation, a gelatine sample spiked with 1 CFU provided enough template DNA for a significant signal.
Conclusion: The potential of a real-time PCR assay for simultaneous detection of B. licheniformis, members of the 'B. cereus group' and B. fumarioli in gelatine is demonstrated.
Significance and Impact of the Study: Implementation of the assay in gelatine producing plants may shorten delivery terms and inform on hazards to public health and suitable remediation procedures.

• 出版日期2004