Asymmetric somatic hybridization between wheat (Triticum aestivum) and Avena sativa L

作者：Xiang FN; Xia GM^*; Chen HM

来源：Science China Life Sciences, 2003, 46(3): 243-252.

• Triticum aestivum
• Avena sativa L
• UV-irradiation
• asymmetric fusion
• hybrids regenerated plant

Protoplasts from cell suspensions of young-embryo-derived, calli, which, were nonregenerable for long-term subculture and protoplasts from embryogenic calli with the regeneration capacity of 75% of the same wheat Jinan 177, were mixed as recipient. Protoplasts from embryogenic calli of Avena sativa (with the regeneration capacity of less than 10%) irradiated with UV at an intensity of 300 muW/cm(2) for 30 s, 1 min, 2 min, 3 min, 5 min were used as the donor. Protoplasts of the recipient and the donor were fused by PEG method. Many. calli and normal green plants were regenerated at high frequency, and were verified as somatic hybrids by chromosome counting, isozyme, 5S rDNA spacer sequence analysis and GISH (genomic in situ hybridization). Fusion combination between protoplasts either from the cell suspensions or from the calli and UV-treated Avena sativa protoplasts could not regenerate green plants.

• 出版日期2003-6
• 单位山东大学