Dietary Whole Glucan Particles Do Not Affect Antibody or Cell-Mediated Immune Responses to Influenza Virus Vaccination in Mice

作者：Pence Brandt D; Hester Shelly N; Donovan Sharon M; Woods Jeffrey A^*

来源：Immunological Investigations, 2012, 41(3): 275-289.

DOI：10.3109/08820139.2011.628732

• Beta-glucan
• Influenza
• Vaccine
• Immunity

Influenza virus is a serious health concern. beta-glucans derived from plants, bacteria, and fungi have been shown to potentiate immune system responses including those elicited by vaccination. However, in these studies beta-glucan was administered as an adjuvant in the vaccine preparation. We hypothesized that addition of a commercially available whole glucan particle supplement to the diet would improve immune response to primary and secondary influenza vaccination in mice. beta-glucan was added to pelleted diet and fed to mice at concentrations designed to deliver 0 (control), 1.8 or 90 mg.kg(-1).day(-1) to each mouse. Influenza vaccine was given intramuscularly in the left hindlimb and primary and secondary responses were assessed. Supplementation with beta-glucan was not effective in boosting immune responses to the vaccine, either in the primary or secondary vaccination experiments. Surprisingly, addition of particulate beta-glucan to the vaccine itself also failed to elicit a greater antibody response. These observations suggest that this particular form of beta-glucan is ineffective in boosting immune response to intramuscular influenza vaccination. Further study is warranted to determine if the use of different mouse models, different vaccine delivery systems, or beta-glucans purified from different strains of bacteria, fungi, or plants could improve outcomes using this or similar protocols.

• 出版日期2012