mTORC2 critically regulates renal potassium handling

作者：Grahammer Florian; Nesterov Viatcheslav; Ahmed Azaz; Steinhardt Frederic; Sandner Lukas; Arnold Frederic; Cordts Tomke; Negrea Silvio; Bertog Marko; Ruegg Marcus A; Hall Michael N; Walz Gerd; Korbmacher Christoph; Artunc Ferruh^*; Huber Tobias B^*

来源：Journal of Clinical Investigation, 2016, 126(5): 1773-1782.

DOI：10.1172/JCI80304

The mTOR pathway orchestrates cellular homeostasis. The rapamycin-sensitive mTOR complex (mTORC1) in the kidney has been widely studied; however, mTORC2 function in renal tubules is poorly characterized. Here, we generated mice lacking mTORC2 in the distal tubule (Rictor(fl/fl) Ksp-Cre mice), which were viable and had no obvious phenotype, except for a 2.5-fold increase in plasma aldosterone. Challenged with a low-Na+ diet, these mice adequately reduced Na+ excretion; however, Rictorfl/fl Ksp-Cre mice rapidly developed hyperkalemia on a high-K+ diet, despite a 10-fold increase in serum aldosterone levels, implying that mTORC2 regulates kaliuresis. Phosphorylation of serum-and glucocorticoid-inducible kinase 1 (SGK1) and PKC-alpha was absent in Rictorfl/fl Ksp-Cre mice, indicating a functional block in K+ secretion activation via ROMK channels. Indeed, patch-clamp experiments on split-open tubular segments from the transition zone of the late connecting tubule and early cortical collecting duct demonstrated that Ba2+-sensitive apical K+ currents were barely detectable in the majority of Rictorfl/fl Ksp-Cre mice. Conversely, epithelial sodium channel (ENaC) activity was largely preserved, suggesting that the reduced ability to maintain K+ homeostasis is the result of impaired apical K+ conductance and not a reduced electrical driving force for K+ secretion. Thus, these data unravel a vital and nonredundant role of mTORC2 for distal tubular K+ handling.

• 出版日期2016-5