Melanin Photosensitization and the Effect of Visible Light on Epithelial Cells

作者：Chiarelli Neto Orlando; Ferreira Alan Silva; Martins Waleska Kerllen; Pavani Christiane; Severino Divinomar; Faiao Flores Fernanda; Maria Engler Silvya Stuchi; Aliprandini Eduardo; Martinez Glaucia R; Di Mascio Paolo; Medeiros Marisa H G; Baptista Mauricio S^*

来源：PLos One, 2014, 9(11): e113266.

DOI：10.1371/journal.pone.0113266

Protecting human skin from sun exposure is a complex issue that involves unclear aspects of the interaction between light and tissue. A persistent misconception is that visible light is safe for the skin, although several lines of evidence suggest otherwise. Here, we show that visible light can damage melanocytes through melanin photosensitization and singlet oxygen (O-1(2)) generation, thus decreasing cell viability, increasing membrane permeability, and causing both DNA photo-oxidation and necro-apoptotic cell death. UVA (355 nm) and visible (532 nm) light photosensitize O-1(2) with similar yields, and pheomelanin is more efficient than eumelanin at generating O-1(2) and resisting photobleaching. Although melanin can protect against the cellular damage induced by UVB, exposure to visible light leads to pre-mutagenic DNA lesions (i.e., Fpg- and Endo III-sensitive modifications); these DNA lesions may be mutagenic and may cause photoaging, as well as other health problems, such as skin cancer.

• 出版日期2014-11-18