H2O2 Biosensor Based on Poly(thionine) and Layer-by-Layer Self-assembly of Carbon Nanotubes and HRP

作者：Yang Shaoming^*; Huang Aihua; Zha Wenling; Wei Zhipeng; Zheng Longzhen

来源：Acta Chimica Sinica, 2011, 69(18): 2143-2147.

• poly(thionine)
• electro-polymerization
• layer-by-layer self-assembly
• biosensor
• H2O2

Carbon nanotubes (CNTs)/horseradish peroxidase (HRP) multilayer reagentless H2O2 biosensor based on poly(thionine) (PTH) mediator was fabricated by the combination of electro-polymerization and layer-by-layer self-assembly method. Electrochemical impedance spectroscopy was used to monitor the layer-by-layer self-assembly process of CNTs/HRP multilayer. The electrochemical behavior of the HRP biosensor was studied by the cyclic voltammetry (CV) and chronoamperometry (I-t). The effect of the number of assembled enzyme layers, work potential, pH value and CNTs were investigated on the response of HRP electrode. The biosensor shows a good linear range of 2.0 X 10(-6)similar to 6.29 X 10(-3) mol/L for H2O2, R=0.998, with a detection limit of 1.0 X 10(-6) mol/L.

• 出版日期2011-9-28
• 单位华东交通大学