alpha-Pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-kappa B-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells

作者：Kang Eunah; Lee Da Hyun; Jung You; Shin Soon Young; Koh Dongsoo^*; Lee Young Han^*

来源：Applied Biological Chemistry, 2016, 59(4): 511-516.

DOI：10.1007/s13765-016-0175-6

• 2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene
• Breast cancer
• Matrix metalloproteinase-9
• Nuclear factor kappa B
• Tumor invasion
• Tumor necrosis factor alpha

2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene (alpha-Pinene) is an organic compound of the terpene class found in the essential oil of many plants. In this study, the inhibitory effect of alpha-pinene on tumor invasion in highly metastatic MDA-MB-231 human breast cancer cells was evaluated. alpha-Pinene inhibited tumor necrosis factor (TNF)-alpha-induced invasiveness of MDA-MB-231 cells as revealed by three-dimensional spheroid invasion assay. Further analysis showed that alpha-pinene reduced TNF alpha-induced matrix metalloproteinase-9 gene promoter activation and mRNA expression in a dose-dependent manner. In addition, alpha-pinene treatment attenuated TNF alpha-induced nuclear factor kappa B (NF-kappa B) activation and NF-kappa B-dependent transcriptional activity. These results suggest that alpha-pinene has a significant effect on the inhibition of tumor invasion and may potentially be developed into an anti-metastatic drug.

• 出版日期2016-8