Mitochondrial Reactive Oxygen Species Modulate Mosquito Susceptibility to Plasmodium Infection

作者：Goncalves Renata L S^*; Oliveira Jose Henrique M; Oliveira Giselle A; Andersen John F; Oliveira Marcus F; Oliveira Pedro L; Barillas Mury Carolina

来源：PLos One, 2012, 7(7): e41083.

DOI：10.1371/journal.pone.0041083

Background: Mitochondria perform multiple roles in cell biology, acting as the site of aerobic energy-transducing pathways and as an important source of reactive oxygen species (ROS) that modulate redox metabolism. %26lt;br%26gt;Methodology/Principal Findings: We demonstrate that a novel member of the mitochondrial transporter protein family, Anopheles gambiae mitochondrial carrier 1 (AgMC1), is required to maintain mitochondrial membrane potential in mosquito midgut cells and modulates epithelial responses to Plasmodium infection. AgMC1 silencing reduces mitochondrial membrane potential, resulting in increased proton-leak and uncoupling of oxidative phosphorylation. These metabolic changes reduce midgut ROS generation and increase A. gambiae susceptibility to Plasmodium infection. %26lt;br%26gt;Conclusion: We provide direct experimental evidence indicating that ROS derived from mitochondria can modulate mosquito epithelial responses to Plasmodium infection.

• 出版日期2012-7-18