Antimicrobial photodynamic therapy with photosensitizer in ethanol improves oxidative status and gingival collagen in a short-term in periodontitis

作者：Pillusky Fernanda Maia; Silva Barcelos Raquel Cristine; Vey Luciana Taschetto; Barin Luisa Machado; Palma Victor de Mello; Maciel Roberto Marinho; Kantorski Karla Zanini; Burger Marilise Escobar; Danesi Cristiane Cademartori

来源：Photodiagnosis and Photodynamic Therapy, 2017, 19: 119-127.

DOI：10.1016/j.pdpdt.2017.05.010

• Periodontal disease
• Type I collagen
• Type III collagen
• Oxidative stress
• Methylene blue

Background: This study evaluated the antimicrobial photodynamic therapy (aPDT) effects using the methylene blue (MB) in ethanol 20% on systemic oxidative status and collagen content from gingiva of rats with periodontitis.
Methods: Rats were divided into five experimental groups: NC (negative control; no periodontitis); PC (positive control; periodontitis without any treatment); SRP (periodontitis and scaling and root planing), aPDT I (periodontitis and SRP + aPDT + MB solubilized in water), and aPDT II (periodontitis and SRP + aPDT + MB solubilized in ethanol 20%). After 7 days of removal of the ligature, the periodontal treatments were performed. At 7/15/30 days, gingival tissue was removed for morphometric analysis. The erythrocytes were used to evaluate systemic oxidative status.
Results: PC group showed higher lipoperoxidation levels at 7/15/30 days. aPDT indicated a protective influence in erythrocytes at 15 days observed by the elevation in levels of systemic antioxidant defense. aPDT II group was the only one that restored the total collagen area in 15 days, and recovered the type I collagen area at the same time point.
Conclusions: aPDT as an adjunct to the SRP can induce the systemic protective response against oxidative stress periodontitis-induced and recover the gingival collagen, thus promoting the healing periodontal, particularly when the MB is dissolved in ethanol 20%.

• 出版日期2017-9