A rubber transferase activator is necessary for natural rubber biosynthesis in dandelion

作者：Epping Janina; van Deenen Nicole; Niephaus Eva; Stolze Anna; Fricke Julia; Huber Claudia; Eisenreich Wolfgang; Twyman Richard M; Pruefer Dirk; Gronover Christian Schulze^*

来源：Nature Plants, 2015, 1(5): UNSP 15048.

DOI：10.1038/NPLANTS.2015.48

High-molecular-mass natural rubber is a valuable plant-derived poly(cis-1,4-isoprene) with many industrial and medical applications. It is synthesized by a rubber cis-prenyltransferase (CPT) complex on the surface of rubber particles in specialized latex-producing cells known as laticifers. Here we show that Taraxacum brevicorniculatum rubber transferase activator (TbRTA), a dandelion homologue of the human Nogo-B receptor, is an essential component of the rubber transferase complex which interacts with rubber CPTs on the surface of rubber particles. The knockdown of TbRTA by RNA interference eliminated rubber biosynthesis, without affecting dolichol accumulation or protein glycosylation in the latex. We also found that TbRTA is localized on the endoplasmic reticulum membrane, supporting the current favoured model of rubber particle biogenesis. We therefore propose that TbRTA acts as a rubber CPT-binding protein that is necessary for the formation of an active rubber transferase complex.

• 出版日期2015-4-27