An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the mitochondrial permeability transition pore

作者：Alavian, Kambiz N.; Beutner, Gisela; Lazrove, Emma; Sacchetti, Silvio; Park, Han-A; Licznerski, Pawel; Li, Hongmei; Nabili, Panah; Hockensmith, Kathryn; Graham, Morven; Porter, George A., Jr.; Jonas, Elizabeth A.^*

来源：Proceedings of the National Academy of Sciences, 2014, 111(29): 10580-10585.

DOI：10.1073/pnas.1401591111

• metabolism
• necrosis
• apoptosis
• ion channel
• excitotoxicity

Mitochondria maintain tight regulation of inner mitochondrial membrane (IMM) permeability to sustain ATP production. Stressful events cause cellular calcium (Ca2+) dysregulation followed by rapid loss of IMM potential known as permeability transition (PT), which produces osmotic shifts, metabolic dysfunction, and cell death. The molecular identity of the mitochondrial PT pore (mPTP) was previously unknown. We show that the purified reconstituted c-subunit ring of the FO of the F1FO ATP synthase forms a voltage-sensitive channel, the persistent opening of which leads to rapid and uncontrolled depolarization of the IMM in cells. Prolonged high matrix Ca2+ enlarges the c-subunit ring and unhooks it from cyclophilin D/cyclosporine A binding sites in the ATP synthase F-1, providing a mechanism for mPTP opening. In contrast, recombinant F-1 beta-subunit applied exogenously to the purified c-subunit enhances the probability of pore closure. Depletion of the c-subunit attenuates Ca2+-induced IMM depolarization and inhibits Ca2+ and reactive oxygen species-induced cell death whereas increasing the expression or single-channel conductance of the c-subunit sensitizes to death. We conclude that a highly regulated c-subunit leak channel is a candidate for the mPTP. Beyond cell death, these findings also imply that increasing the probability of c-subunit channel closure in a healthy cell will enhance IMM coupling and increase cellular metabolic efficiency.

• 出版日期2014-7-22