Model Peptides Uncover the Role of the beta-Secretase Transmembrane Sequence in Metal Ion Mediated Oligomerization

作者：Munter Lisa M; Sieg Holger; Bethge Tobias; Liebsch Filip; Bierkandt Frank S; Schleeger Michael; Bittner Heiko J; Heberle Joachim; Jakubowski Norbert; Hildebrand Peter W; Multhaup Gerd^*

来源：Journal of the American Chemical Society, 2013, 135(51): 19354-19361.

DOI：10.1021/ja410812r

The P-secretase or beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) is the enzyme responsible for the formation of amyloid-beta peptides, which have a major role in Alzheimer pathogenesis. BACE1 has a transmembrane sequence (TMS), which makes it unique among related proteases. We noticed that the BACE1 TMS contains an uncommon sulfur-rich motif. The sequence MxxxCxxxMxxxCxMxC spans the entire TMS, resembles metal ion binding motifs, and is highly conserved among homologues. We used a synthetic 31-mer model peptide comprising the TMS to study metal ion binding and oligomerization. Applying diverse biochemical and biophysical techniques, we detected dimer and trimer formation of the TMS peptide with copper ions. Replacement of the central Cys466 by Ala essentially abolished these effects. We show that the peptide undergoes a redox reaction with copper ions resulting in a disulfide bridge involving Cys466. Further, we find peptide trimerization that depends on the presence of monovalent copper ions and the sulfhydryl group of Cys466. We identified Cys466 as a key residue for metal ion chelation and to be the core of an oligomerization motif of the BACE1-TMS peptide. Our results demonstrate a novel metal ion controlled oligomerization of the BACE1 TMS, which could have an enormous therapeutic importance against Alzheimer disease.

• 出版日期2013-12-25
• 单位河北医科大学