AFM study of adsorption of protein A on a poly(dimethylsiloxane) surface

作者：Yu Ling; Lu Zhisong; Gan Ye; Liu Yingshuai; Li Chang Ming^*

来源：Nanotechnology, 2009, 20(28): 285101.

DOI：10.1088/0957-4484/20/28/285101

In this paper, the morphology and kinetics of adsorption of protein A on a PDMS surface is studied by AFM. The results of effects of pH, protein concentration and contact time of the adsorption reveal that the morphology of adsorbed protein A is significantly affected by pH and adsorbed surface concentration, in which the pH away from the isoelectric point (IEP) of protein A could produce electrical repulsion to change the protein conformation, while the high adsorbed surface protein volume results in molecular networks. Protein A can form an adsorbed protein film on PDMS with a maximum volume of 2.45 x 10(-3) mu m(3). This work enhances our fundamental understanding of protein A adsorption on PDMS, a frequently used substrate component in miniaturized immunoassay devices.

• 出版日期2009-7-15
• 单位南阳理工学院