Molecular Cloning and Identification of Novel ω-gliadin Genes from Triticum Species

作者：Li, Z. L.; Wu, D. D.; Li, H. Y.; Chen, G.; Cao, W. G.; Ning, S. Z.; Liu, D. C.; Zhang, L. Q.^*

来源：Cereal Research Communications, 2016, 44(2): 309-319.

DOI：10.1556/0806.44.2016.020

• omega-gliadin
• Triticum
• cloning
• pseudogene

Gliadin is a main component of gluten proteins that affect functional properties of bread making and contributes to the viscous nature of doughs. In this study, thirteen novel omega-gliadin genes were identified in several Triticum species, which encode the ARH-, ATD- and ATN-type proteins. Two novel types of omega-gliadins: ATD- and ATN-have not yet been reported. The lengths of 13 sequences were ranged from 927 to 1269 bp and the deduced mature proteins were varied from 309 to 414 residues. All 13 genes were pseudogenes because of the presence of internal stop codons. The primary structure of these.-gliadin genes included a signal peptide, a conserved N-terminal domain, a repetitive domain and a conserved C-terminus. In this paper, we first characterize omega-gliadin genes from T. timo-pheevi ssp. timopheevi and T. timopheevi ssp. araraticum. The omega-gliadin gene variation and the evolutionary relationship of omega-gliadin family genes were also discussed.

• 出版日期2016-6
• 单位四川农业大学