APRIL knockdown suppresses migration and invasion of human colon carcinoma cells

作者：Ding Weifeng; Wang Jinchun; Sun Baolan; Ju Shaoqing; Yuan Hongxiang; Wang Xing; Wang Yueguo; Wang Huimin^*

来源：Clinical Biochemistry, 2009, 42(16-17): 1694-1698.

DOI：10.1016/j.clinbiochem.2009.06.019

• APRIL
• RNAi
• Invasion
• Migration
• Colorectal neoplasms

Objective: The purpose of this study was to investigate the function of a proliferation-inducing ligand (APRIL) gene among the metastatic colorectal cancer (CRC) disease.
Methods: Cell adhesion, cell migration and invasion behavior were detected after knockdown of APRIL expression in the colon carcinoma cell line, SW480 by RNAi and the rescue experiments were performed with recombinant human APRIL (rhAPRIL) in vitro.
Results: This original study indicated that knockdown of APRIL expression strongly inhibited colon carcinoma cell adhesion, cell migration and invasion in vitro. By contrast, reconstitution of APRIL expression with rhAPRIL in these APRILi cells, prominently restores CRC cell migration and invasion.
Conclusion: These results strongly suggest that APRIL play an important role in the tumor development of the invasive or migratory behavior of CRC cells and may be a useful therapeutic target in the malignant colon carcinomas.

• 出版日期2009-11
• 单位南通大学