科研之友
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摘要
Background: The housefly, Musca domestica, has developed resistance to most insecticides applied for its control. Expression of genes coding for detoxification enzymes play a role in the response of the housefly when encountered by a xenobiotic. The highest level of constitutive gene expression of nine P450 genes was previously found in a newly-collected susceptible field population in comparison to three insecticide-resistant laboratory strains and a laboratory reference strain. Results: We compared gene expression of five P450s by qPCR as well as global gene expression by RNAseq in the newly-acquired field population (845b) in generation F-1, F-13 and F-29 to test how gene expression changes following laboratory adaption. Four (CYP6A1, CYP6A36, CYP6D3, CYP6G4) of five investigated P450 genes adapted to breeding by decreasing expression. CYP6D1 showed higher female expression in F-29 than in F-1. For males, about half of the genes accessed in the global gene expression were up-regulated in F-13 and F-29 in comparison with the F-1 population. In females, 60% of the genes were up-regulated in F-13 in comparison with F-1, while 33% were up-regulated in F-29. Forty potential P450 genes were identified. In most cases, P450 gene expression was decreased in F-13 flies in comparison with F-1. Gene expression then increased from F-13 to F-29 in males and decreased further in females. Conclusion: The global gene expression changes massively during adaptation to laboratory breeding. In general, global expression decreased as a result of laboratory adaption in males, while female expression was not unidirectional. Expression of P450 genes was in general down-regulated as a result of laboratory adaption. Expression of hexamerin, coding for a storage protein was increased, while gene expression of genes coding for amylases decreased. This suggests a major impact of the surrounding environment on gene response to xenobiotics and genetic composition of housefly strains.
- 出版日期2014-1-28
