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摘要
Objectives: In this study, the effects of adalimumab (ADA), a fully humanized IgG1 monoclonal antibody to tumor necrosis factor alpha, on experimentally acute pancreatitis (AP) were examined.
Methods: Healthy Wistar rats (n = 32) were randomly divided into 4 groups: group 1, AP; group 2, AP + ADA; group 3, control (physiologic saline), and group 4, physiologic saline + ADA (n = 8/group). Acute pancreatitis was induced with a retrograde injection of 3% sodium (Na)- taurocholate into the common biliopancreatic duct. Adalimumab was simultaneously administered at 50 mg/kg intraperitoneally for groups 2 and 4. Physiologic saline was administered instead of Na-taurocholate for non-AP groups. After 24 hours, serum amylase, lactate dehydrogenase, pancreatic myeloperoxidase, and malondialdehyde activities, along with pancreatic histopathology, were examined.
Results: Adalimumab treatment significantly decreased serum amylase activity (AP, 2778.25 +/- 298.80; AP + ADA, 2143.13 +/- 221.69; control, 1541.00 +/- 148.39; ADA, 1143.00 +/- 256.30 U/L; P < 0.001), lactate dehydrogenase activity (AP, 2978.37 +/- 364.65; AP + ADA, 2582.75 T 164.23; control 931.25 +/- 135.93; ADA, 582.62 +/- 99.37 U/L; P < 0.001), myeloperoxidase activity (AP, 1.44 +/- 0.20; AP + ADA, 0.86 +/- 0.01; control, 0.60 +/- 0.17; ADA, 0.41 +/- 0.00 U/g of wet tissue; P < 0.001), malondialdehyde activity (AP, 16.94 +/- 3.98; AP + ADA, 7.66 +/- 2.27; control, 9.07 +/- 1.00; ADA, 3.58 +/- 0.30 nmol/g; P < 0.01), and total histopathologic scores (AP, 2.75 +/- 0.16; AP + ADA, 1.50 +/- 0.19; control, 0.00 +/- 0.00; ADA, 0.00 +/- 0.00; P < 0.001).
Conclusions: These results support the idea that adalimumab might be beneficial for severity of AP.
- 出版日期2010-11
