ALTERATIONS IN CELL-CELL COMMUNICATION IN HUMAN PAPILLOMAVIRUS TYPE-16 (HPV16) TRANSFORMED RAT MYOBLASTS

作者：ENNAJI MM^*; SCHWARTZ JL; MEALING G; BELBARAKA L; PARKER C; PARENTAUX M; JOUISHOMME H; ARELLA M; WHITFIELD JF; PHIPPS J

来源：Cellular and Molecular Biology, 1995, 41(4): 481-498.

• HPV 16
• CELL-CELL COMMUNICATION
• GAP JUNCTION
• CONNEXIN 43
• GJIC

A reduction of gap-junctional intercellular communication (GJIC) often accompanies neoplastic transformation. The present work demonstrates that transformation by the oncogenic human DNA virus, human papilloma virus 16 (HPV16), also reduces GJIC between L(6) rat myoblasts. HPVs are associated with anogenital cancers, the incidence of which is increasing in HIV positive patients of both sexes. Using videofluorescence imaging of Fura-2 loaded cells a lack of GJIC between transformed HPV16-L(6) cells was first indicated by uncoordinated brief [Ca2+](i) spikes in clusters of DMSO-treated HPV16-L(6) cells instead of the synchronous, sustained [Ca2+](i) surges in clusters of DMSO-treated L(6) cells. Reduced GJIC between HPV16-L(6) cells was demonstrated directly by a much reduced transfer of lucifer yellow dye from HPV16-L(6) cells, which had been loaded with the dye through electroporation with an EPIZAP II in situ electroporator, to neighbouring non-electroporated HPV 16-L(6) cells. One reason for this reduced GJIC between HPV 16-L(6) cells could have been their dramatically enhanced activity of membrane-associated PKC which is known to phosphorylate connexins and down-regulate gap junctions. However, the main reason was the viral-induced inhibition of the expression of a major gap junction component, Cx43 (Connexin 43), in the transformed myoblasts.

• 出版日期1995-6