Adhesion of Human U937 Monocytes to Nitrogen-Rich Organic Thin Films: Novel Insights into the Mechanism of Cellular Adhesion

作者：Girard Lauriault Pierre Luc; Truica Marasescu Florina; Petit Alain; Wang Hong T; Desjardins Patrick; Antoniou John; Mwale Fackson^*; Wertheimer Michael R

来源：Macromolecular Bioscience, 2009, 9(9): 911-921.

DOI：10.1002/mabi.200800359

• biomaterials
• cell adhesion
• gene expression
• plasma polymerisation
• primary amines

We present a two-fold study designed to elucidate the adhesion mechanism of human U937 monocytes on novel N-rich thin films deposited by plasma- and VUV photo-polymerisation, so-called "PVP:N" materials. It is shown that there exist sharply-defined ("critical") surface-chemical conditions that are necessary to induce cell adhesion. By comparing the film chemistries at the "critical" conditions, we demonstrate the dominant role of primary amines in the cell adhesion mechanism. Ouantitative real-time RT-PCR experiments using U937 cells that had adhered to PVP:N materials for up to 24h are presented, The adhesion induces a transient expression of cytokines, markers of macrophage activation, as well as a more sustained expression of PPAR gamma and ICAM-I.

• 出版日期2009-9-9
• 单位河北医科大学