A novel electrochemical DNA-sensing nanoplatform based on supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels

作者：Kazerooni Hanif; Nasernejad Bahram^*

来源：Journal of Applied Electrochemistry, 2015, 45(12): 1289-1298.

DOI：10.1007/s10800-015-0891-3

• Biosensors
• Electrochemical sensors
• Nanotechnology
• Nucleic acids
• Polymers

In this paper, the application of supramolecular ionic liquids grafted on nitrogen-doped graphene aerogels (SIL-g-(N)GAs), a novel electrode system, for the preparation of electrochemical DNA sensing platform is proposed. The super dispersion of SIL-g-(N)GAs in water makes it an ideal candidate for biological purposes such as gene delivery. In fact, SIL-g-(N)GAs/glassy carbon working electrode (GCE) can realize the simultaneous detection of all four DNA bases in double-stranded DNA without a prehydrolysis step. On the SIL-g-(N)GAs/GCE, due to the presence of SIL on the surface of three-dimensional nitrogen-doped graphene, the anchoring of the DNA probe can be achieved by electrostatic association of SIL cations with the DNA backbone readily. So, herein we report a novel strategy for DNA hybridization without any electroactive tags or intercalators and suggest the potential applications of SIL-g-(N)GAs/GCE in the label-free electrochemical detection of DNA hybridization or DNA damage for further research. Moreover, the influence of potentially interfering substances on the determination of DNA is investigated.

• 出版日期2015-12