Diversity of Sinorhizobium meliloti and S-Medicae nodulating Medicago truncatula according to host and soil origins

作者：Zribi K; Mhamdi R; Huguet T; Aouani ME^*

来源：World Journal of Microbiology and Biotechnology, 2005, 21(6-7): 1009-1015.

DOI：10.1007/s11274-004-7653-4

• genetic diversity
• Medicago truncatula
• plant host population
• repetitive extragenic palindromic-polymerase chain reaction (REP-PCR)
• Sinorhizobium medicae
• Sinorhizobium meliloti
• soil

The model legume Medicago truncatula was used to trap natural populations of Sinorhizobium meliloti and Sinorhizobium medicae in Tunisian soils to explore their genetic diversity. About 155 Sinorhizobium isolates were trapped from a combination of three soils and four Medicago truncatula populations in order to analyse soil and plant population effects on nodulating Sinorhizobium diversity. The species assignment was done according to the restriction fragment length polymorphism analysis of polymerase chain reaction (PCR/RFLP) of 16S rRNA genes and their infraspecific genetic diversity was assessed with the repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) technique. It appeared that the trapped bacteria were clustered according to the soil of origin, particularly Sinorhizobium medicae isolates. However, regarding the plant population effect, it appeared that no major clustering tendency could be suggested even if the Bulla Regia and Soliman Medicago truncatula populations appeared to nodulate together specific Sinorhizobium medicae genotypes.

• 出版日期2005-10