Measuring the effect of ligand binding on the interface stability of multimeric proteins using dynamic light scattering

作者：Marion James D; Van Danielle N; Bell J Ellis; Bell Jessica K^*

来源：Analytical Biochemistry, 2010, 407(2): 278-280.

DOI：10.1016/j.ab.2010.08.019

We have demonstrated that an approach using guanidine hydrochloride at low concentrations to progressively disrupt protein-protein interactions can be quantitated using dynamic light scattering. This approach is sensitive enough to detect ligand-induced changes of subunit-subunit interactions for homo-hexameric glutamate dehydrogenase, allowing Delta Delta G of reversible subunit dissociation to be calculated. The use of dynamic light scattering makes this approach generally applicable to soluble proteins to monitor the relative strength of protein-protein interactions with a particular emphasis on assessing the impact of ligand binding on such interfaces.

• 出版日期2010-12-15