PMI (manA) as a nonantibiotic selectable marker gene in plant biotechnology

作者：Stoykova P^*; Stoeva Popova P

来源：Plant Cell, Tissue and Organ Culture, 2011, 105(2): 141-148.

DOI：10.1007/s11240-010-9858-6

• Mannose
• Phosphomannose isomerase
• Positive selection
• Plant genetic transformation

Due to the rising public concern over the use of genes conferring antibiotic and herbicide resistance, alternative systems for selection after plant transformation are being developed. A positive selection system consists of a physiologically inert metabolite as the selection agent and a respective gene which determines a metabolic advantage via selection agent utilization. The transformed cells are able to overcome the suppressive effects of the selection, while the untransformed ones starve but are not killed. The enzyme phosphomannose isomerase (PMI, E.C. 5.3.1.8) catalyzes the reversible interconversion of mannose-6-phosphate and fructose-6-phosphate in prokaryotic and eukaryotic organisms. The PMI selection system is called positive due to the effect of "starvation" caused to the nontransformed plant tissue because of its incapability to utilize mannose as a carbon source. In this mini-review we researched the literature to obtain a more detailed view of the characteristics, specifics, problems, and advantages of applying the PMI/mannose selection system.

• 出版日期2011-5