Ultrasonic and free-radical degradation of mannan from Candida albicans

作者：Cizova Alzbeta^*; Bystricky Peter; Bystricky Slavomir

来源：International Journal of Biological Macromolecules, 2015, 75: 32-36.

DOI：10.1016/j.ijbiomac.2014.12.046

• Mannan
• Candida albicans
• Ultrasound
• Degradation
• Fenton reaction

Mannan from pathogenic Candida albicans serotype A was degraded by means of ultrasound and/or OH. generated in situ by Fenton reaction. The kinetics of degradation was monitored by HPLC analysis and the weight-average molecular weights (M-w) and index of polydispersity (PDI) were compared. A well-defined low-molecular-weight mannan (similar to 30 kDa) with narrowed PDI of 1.8 was obtained after 120 mm of ultrasonication. Similar or even lower Mw (up to 16 kDa) was achieved upon free-radical exposure depending on Fe2+ concentration used; however, this was accompanied by overall broadening of PDI and distinct changes in polymer structure as indicated by NMR analysis.

• 出版日期2015-4