A sensitive electrochemical immunosensor for label-free detection of Zika-virus protein

作者：Kaushik Ajeet; Yndart Adriana; Kumar Sanjeev; Jayant Rahul Dev; Vashist Arti; Brown Ashley N; Li Chen Zhong; Nair Madhavan^*

来源：Scientific Reports, 2018, 8(1): 9700.

DOI：10.1038/s41598-018-28035-3

This work, as a proof of principle, presents a sensitive and selective electrochemical immunosensor for Zika-virus (ZIKV)-protein detection using a functionalized interdigitated micro-electrode of gold (IDE-Au) array. A miniaturized IDE-Au immunosensing chip was prepared via immobilization of ZIKV specific envelop protein antibody (Zev-Abs) onto dithiobis(succinimidyl propionate) i.e., (DTSP) functionalized IDE-Au (electrode gap/width of 10 mu m). Electrochemical impedance spectroscopy (EIS) was performed to measure the electrical response of developed sensing chip as a function of ZIKV-protein concentrations. The results of EIS studies confirmed that sensing chip detected ZIKV-protein selectively and exhibited a detection range from 10 pM to 1 nM and a detection limit of 10 pM along with a high sensitivity of 12 k Omega M(-1). Such developed ZIKV immune-sensing chip can be integrated with a miniaturized potentiostat (MP)-interfaced with a smartphone for rapid ZIKV-infection detection required for early stage diagnostics at point-of-care application.

• 出版日期2018-6-26