Assessment of shikonin for potential estrogenic activity by dual-luciferase reporter based bioluminescent measurements in vitro

作者：Zhang, Yi; Li, Ping-Ping^*

来源：Pharmazie, 2011, 66(2): 141-143.

DOI：10.1691/ph.2011.0714

• STEROID SULFATASE GENES
• LITHOSPERMUM-ERYTHRORHIZON
• CELLS
• PHARMACOLOGY
• ACTIVATION
• MECHANISMS
• EXPRESSION
• EXTRACT
• CANCER

Shikonin, an active component of Lithospermum erythrorhizon Sieb. et Zucc., shows multiple pharmacological properties. However, the estrogenic activity of shikonin is remaining unclear. We assessed the potential estrogenic activity of shikonin with dual-luciferase reporter assay and bioluminescent measurements, by using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells. Estrogenic activity of shikonin, even at high concentration did not alter significantly compared to negative control (p > 0.05) and were significantly lower than those with E-2 (p < 0.01). Concluding, shikonin demonstrates no estrogenic activity in vitro.

• 出版日期2011-2
• 单位北京市肿瘤防治研究所; 北京大学; 北京中医药大学