A new technique for the radiolabelling of mixed leukocytes with zirconium-89 for inflammation imaging with positron emission tomography

作者：Fairclough M^*; Prenant C; Ellis B; Boutin H; McMahon A; Brown G; Locatelli P; Jones A K P

来源：Journal of Labelled Compounds and Radiopharmaceuticals, 2016, 59(7): 270-276.

DOI：10.1002/jlcr.3392

• PET
• Zr-89
• Cu-64
• inflammation imaging
• white blood cell trafficking
• chitosan nanoparticles

Mixed leukocyte (white blood cells [WBCs]) trafficking using positron emission tomography (PET) is receiving growing interest to diagnose and monitor inflammatory conditions. PET, a high sensitivity molecular imaging technique, allows precise quantification of the signal produced from radiolabelled moieties. We have evaluated a new method for radiolabelling WBCs with either zirconium-89 (Zr-89) or copper-64 (Cu-64) for PET imaging. Chitosan nanoparticles (CNs) were produced by a process of ionotropic gelation and used to deliver radiometals into WBCs. Experiments were carried out using mixed WBCs freshly isolated from whole human blood. WBCs radiolabelling efficiency was higher with [Zr-89]-loaded CN (76.8 +/- 9.6% (n=12)) than with [Cu-64]-loaded CN (26.3 +/- 7.0 % (n=7)). [Zr-89]-WBCs showed an initial loss of 28.4 +/- 5.8% (n=2) of the radioactivity after 2h. This loss was then followed by a plateau as Zr-89 remains stable in the cells. [Cu-64]-WBCs showed a loss of 85 +/- 6% (n=3) of the radioactivity after 1h, which increased to 96 +/- 6% (n=3) loss after 3h. WBC labelling with [Zr-89]-loaded CN showed a fast kinetic of leukocyte association, high labelling efficiency and a relatively good retention of the radioactivity. This method using Zr-89 has a potential application for PET imaging of inflammation.

• 出版日期2016-6-15