An experimental and theoretical study of the amino acid side chain Raman bands in proteins

作者：Sjoeberg Beatrice; Foley Sarah^*; Cardey Bruno; Enescu Mironel

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2014, 128: 300-311.

DOI：10.1016/j.saa.2014.02.080

• Raman Spectroscopy
• DFT calculations
• Tripeptides
• Bovine serum albumin
• Betalactoglobulin
• Lysozyme

The Raman spectra of a series of tripeptides with the basic formula GlyAAGly where the central amino acid (AA) was tryptophan, tyrosine, phenylalanine, glycine, methionine, histidine, lysine and leucine were measured in H2O. The theoretical Raman spectra obtained using density functional theory (DFT) calculations at the B3LYP/6-311+G(2df,2pd) level of theory allows a precise attribution of the vibrational bands. The experimental results show that there is a blue shift in the frequencies of several bands of the amino acid side chains in tripeptides compared to free amino acids, especially in the case of AAs containing aromatic rings. On the other hand, a very good agreement was found between the Raman bands of AA residues in tripeptides and those measured on three model proteins: bovine serum albumin, beta-lactoglobulin and lysozyme. The present analysis contributes to an unambiguous interpretation of the protein Raman spectra that is useful in monitoring the biological reactions involving AA side chains alteration.

• 出版日期2014-7-15