A Collection of Popcorn as a Reservoir of Genes for the Generation of Lineages

作者：Nunes de Carvalho Misael Severino; Mangolin Claudete Aparecida; Scapim Carlos Alberto; da Silva Teresa Aparecida; Pires da Silva Machado Maria de Fatima

来源：Molecular Biotechnology, 2013, 53(3): 300-307.

DOI：10.1007/s12033-012-9527-6

• Popcorn
• SSR locus
• Microsatellite
• Genetic diversity

In the present study, we analyze the genetic structure and diversity among accessions of popcorn obtained from the CIMMYT International Maize and Wheat Improvement Center that represent the diversity available for current use by breeding programs. The main objectives were to identify SSR loci that were the best indicators of genetic diversity, to measure the genetic diversity within popcorn genotypes, and to analyze the genetic structure of the promising populations destined for use in breeding programs. The mean gene diversity of all SSR loci was 0.6352. An extremely high population differentiation level was detected (F (st) = 0.3152) with F (st) for each locus ranging from 0.1125 (Umc1229) to 0.4870 (Umc1755). Analyzing the genetic structure of eight popcorn accessions was especially important for identifying both SSR loci with high levels of heterozygosity and genotypes showing high heterozygosity (Boya462 and Arzm13 050). This analysis should be the medium and long-term selection goal for the generation of inbred lines and the future production of new cultivars. Plant accessions Arzm05 083, Arzm13 050, and Urug298 may also be useful varieties that exhibit important agronomic characteristics that can be used through crosses to broaden the genetic basis of popcorn.

• 出版日期2013-3