A novel matrix for high performance affinity chromatography and its application in the purification of antithrombin III

作者：Zhao R^*; Luo H; Shangguan D; Liu GQ

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2005, 816(1-2): 175-181.

DOI：10.1016/j.jchromb.2004.11.030

• viscose fiber
• high performance affinity chromatography
• heparin
• antithrombin III

Viscose fiber, a regenerated cellulose, was evaluated for using as a novel matrix for high performance affinity chromatography. With a one-step activation with epichlorohydrin, heparin can be readily covalently attached to the matrix. This heparin-viscose fiber material was used for purifying antithrombin III (AT III) from human plasma. The purity of the AT III from this one-step purification is 93% as measured by SDS-PAGE and the protein recovery yield is about 90%. This column is highly specific as described by the dissociation constant of the complex of immobilized heparin and AT III, which was 2.83 x 10(-5) mol/L. And more important, this viscose fiber material demonstrated its excellent mechanical property that allows the flow rate to reach up to 900 cm/h or more.

• 出版日期2005-2-25
• 单位中国科学院