Adsorption and Electrooxidation of Nucleic Acids at Glassy Carbon Electrodes Modified with Multiwalled Carbon Nanotubes Dispersed In Polylysine

作者：Jalit Yamile; Moreno Monica; Gutierrez Fabiana A; Sanchez Arribas Alberto; Chicharro Manuel; Bermejo Esperanza; Zapardiel Antonio; Parrado Concepcion; Rivas Gustavo A^*; Rodriguez Marcela C

来源：Electroanalysis, 2013, 25(5): 1116-1121.

DOI：10.1002/elan.201200622

• Carbon nanotubes
• Genosensors
• Hybridization electrochemical biosensors
• DNA adsorption
• Polylysine
• Carbon nanotubes dispersion

We report the advantages of the adsorption and electrooxidation of oligonucleotides and calf-thymus double stranded DNA (dsDNA) at glassy carbon electrodes (GCE) modified with a dispersion of multiwalled carbon nanotubes (MWCNT) in poly-L-lysine (Plys) (GCE/MWCNT-Plys). Important enhancement in the guanine oxidation signal was obtained by adsorptive stripping voltammetry (AdSV) due to a most favorable interaction between the negatively charged DNAs and the positively charged Plys that support the MWCNT. The layer of oligo(dG)11 immobilized at GCE/MWCNT-Plys was successfully used for the selective detection of the hybridization event using oligo(dG)11/oligo(dC)11 as model.

• 出版日期2013-5