Mechanisms of tomato mosaic virus RNA replication and its inhibition by the host resistance factor Tm-1

作者：Ishibashi Kazuhiro^*; Ishikawa Masayuki

来源：Current Opinion in Virology, 2014, 9: 8-13.

DOI：10.1016/j.coviro.2014.08.005

In the plant immune system, sensor proteins encoded by dominant resistance genes activate a defense response upon pathogen infection. The tomato mosaic virus (ToMV) resistance gene Tm-1 is exceptional in that it inhibits ToMV multiplication without inducing a defense response. Several lines of evidence had suggested that Tm-1 encodes a direct inhibitor of ToMV RNA replication. The Tm-1 gene product was identified by purification of an inhibitor protein using a cell-free translation and replication system for ToMV RNA. Further analyses using the system showed that Tm-1 bound ToMV replication proteins, and that the Tm-1-bound ToMV replication proteins retained the ability to bind membranes, while Tm-1 inhibited replication complex formation on the membranes.

• 出版日期2014-12