Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis

作者：Na Di; Liu Fu Nan; Miao Zhi Feng; Du Zong Min; Xu Hui Mian^*

来源：World Journal of Gastroenterology, 2009, 15(5): 570-577.

DOI：10.3748/wjg.15.570

• Peritoneal carcinomatosis
• Stomach neoplasm
• Astragalus plant
• Mesothelial cell
• Apoptosis

AIM: To determine the inhibitory effect of Astragalus memebranaceushas on gastric cancer cell supernatant-induced apoptosis of human peritoneal mesothelial cells.
METHODS: Human peritoneal mesothelial cell (HPMC) line HMrSV5 was co-incubated with gastric cancer cell supernatant (MKN45) and/or Astragalus memebranaceushas. Morphological changes in gastric cancer cells were observed under phase-contrast microscope. Quantitative cell damage was determined by MTT assay. Apoptosis was determined under transmission electron microscope and quantified by deteching acridine orange/ethidium bromide-stained (AO/EB) condensed nuclei under fluorescent microscope or by flow cytometry. Expressions of Bcl-2 and Bax were evaluated with immunostaining.
RESULTS: Morphological changes and exfoliation occurred and naked areas appeared in cultured HMrSV5 cells 24 h after they were treated with gastric cancer cell supernatant. Cell supernatant from gastric cancer cell MKN45 induced apoptosis of HMrSV5 cells in a time-dependent manner. Obvious morphological changes were observed in cell apoptosis, such as condensation of chromatin, nuclear fragmentations and apoptotic bodies. Astragalus memebranaceus could partly suppress these changes and regulate the expressions of Bcl-2 and Bax in HMrSV5 cells.
CONCLUSION: Gastric cancer cells induce apoptosis of HPMC through its supernatant. Astragalus memebranaceushas inhibits this kind of phenomenon and therefore, can be used an adjuvant chemotherapeutic agent in gastric cancer therapy.

• 出版日期2009-2-7
• 单位中国医科大学