Neuraminidase enhances the initial steps of human T-cell leukemia virus type 1 replication

作者：Tanaka Masakazu; Sun Binlian; Tezuka Kenta; Fujisawa Jun ichi; Tanaka Yuetsu; Hoshino Hiroo; Miwa Masanao^*

来源：Microbes and Infection, 2010, 12(2): 119-125.

DOI：10.1016/j.micinf.2009.10.003

• HTLV-1 infection
• Neuraminidase
• Cell-free virus

Human T-cell leukemia virus type 1 (HTLV-1) infection is involved in the development of adult T-cell leukemia and HTLV-1-associated myelopathy/tropical spastic paraparesis. A high HTLV-1 proviral load in circulating lymphocytes of HTLV-1 carriers is a risk factor for HTLV-1-related diseases. The virus-cell interaction is linked to viral tropism and pathogenesis. Characterization of the factors that affect HTLV-1 infection is important for preventing HTLV-1 infection. HTLV-1 virions are believed to be weakly infectious under cell culture conditions; however, we found that the treatment of HTLV-1 virions with microbial neuraminidase, an enzyme catalyzing the removal of sialic acid residues from various glycoconjugates, enhanced the number of proviral DNAs in infected cells in a dose-dependent manner. Neuraminidase treatment of virions, but not target cells, enhanced viral binding and entry into cells and viral infectivity; treatment of target cells prior to infection had no effect. Moreover, the number of HTLV-1-mediated syncytia was higher in the presence of neuraminidase. Our results suggest a possible contribution of microbial agents carrying neuraminidase activity to HTLV-1 pathogenesis.

• 出版日期2010-2