Application of Principal Component Analysis for evaluation of chemical and antimicrobial properties of honey bee (Apis mellifera) venom

作者：Kokot Zenon J^*; Matysiak Jan; Klos Jolanta; Kedzia Bogdan; Holderna Kedzia Elzbieta

来源：Journal of Apicultural Research, 2009, 48(3): 168-175.

DOI：10.3896/IBRA.1.48.3.04

• honey bee venom
• High Performance Liquid Chromatography
• HPLC
• Principal Component Analysis
• PCA
• Minimum Inhibitory Concentration
• MIC

The aim of this study was to employ Principal Component Analysis (PCA) to clarify the general distribution patterns or similarities of four major constituents (apamine, phospholipase A(2), mast cell degranulating peptide, and melittin) of bee venom collected from two honey bee strains in various months and years. The contents of these main bee venom components have been analyzed using High Performance Liquid Chromatography (HPLC). In addition, the antimicrobial activity of the product was characterized by determination of the Minimum Inhibitory Concentration (MIC). Using PCA it might be possible to find and describe the correlation between the constituents of bee venom and their antibacterial activity. This is probably the first study in which the composition and activity of bee venom has been compared applying such approach. It was shown that the strain of bee appears to be the only criteria for bee venom sample classification. Moreover, a strong correlation between phospholipase A(2) and melittin was confirmed. PCA analysis showed that there is a weak relationship between the analyzed honey bee venom constituents and MIC values. Minor components of bee venom therefore probably also significantly influence its antimicrobial activity.

• 出版日期2009
• 单位河北医科大学