A data treatment method for detecting fluorescence anisotropy peaks in capillary electropherograms

作者：Picou Ryan A; Kheterpal Indu; Gilman S Douglass^*

来源：Analytica Chimica Acta, 2012, 739: 99-103.

DOI：10.1016/j.aca.2012.06.023

• Fluorescence anisotropy
• Data treatment
• Capillary electrophoresis
• Amyloid beta peptide

A data treatment method is presented to detect fluorescence anisotropy (FA) peaks in capillary electrophoresis electropherograms. The data treatment method converts plots of fluorescence anisotropy vs. time that contain no peaks that are distinguishable from the noise of the anisotropy background into plots that show distinct fluorescence anisotropy peaks. The method was demonstrated using laser-induced fluorescence anisotropy data from individual A beta (1-42) aggregates separated using capillary electrophoresis. Applying this data treatment method enabled the detection of anisotropy peaks for individual A beta aggregate fluorescence peaks that were not observed prior to the data treatment method. The data treatment method is not specifically designed for A beta aggregate analysis or capillary electrophoresis, and it should be applicable to other applications and other separation methods with FA detection.

• 出版日期2012-8-20