Direct quantitative measurement of the kinetics of HLA-specific antibody interactions with isolated HLA proteins

作者：Daga Sunil; Moyse Harry; Briggs David; Lowe David; Evans Neil; Jones James; Buchli Rico; McMurtrey Curtis; Mulder Arend; Hildebrand William; Claas Frans; Higgins Robert; Mitchell Daniel A; Zehnder Daniel

来源：Human Immunology, 2018, 79(2): 122-128.

DOI：10.1016/j.humimm.2017.10.012

• HLA-specific antibodies
• Affinity
• Surface plasmon resonance
• Binding kinetics
• Epitope
• Eplet

HLA specific antibodies vary in their pathogenicity and this is likely to be the net effect of constant chain usage, quantity, specificity, and affinity. Here we have measured the affinity of human monoclonal antibodies for a range of HLA proteins. Purified antibodies and ligands allowed dynamic interactions to be measured directly by surface plasmon resonance. Physiochemical differences between pairs of ligands were quantified using electrostatic mismatch and hydrophobic mismatch scores. All antibodies were characterized by fast on -rates and slow off rates but with a wide range of association rates (k(on), 3.63-24.25 x 10(5) per mol per second) and dissociation rates (k(off), 0.99-10.93 x 10(-3) per second). Dissociation constants (K-D.) ranged from 5.9 x 10(-10) M to 3.0 x 10(-8)M. SN320G6 has approximately a twentyfold greater affinity for HLA A2 compared with SN607D8, but has a similar affinity for HLA-A2 and B57. In contrast, SN607D8 has greater than a twofold greater affinity for HLA-A2 compared with A68. Similarly, WK1D12 has about a threefold greater affinity for HLA-B27 compared with B7. The higher affinity interactions correlate with the specificity of stimulating antigen. This is the first study to directly measure the binding kinetics and affinity constants for human alloantibodies against HLA.

• 出版日期2018-2