科研之友
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摘要
More than 250 toxic substances, including pesticides and mycotoxins, have been identified and quantified in ginkgo biloba nutraceutical products using liquid chromatography (LC) coupled to high resolution mass spectrometry (Exactive-Orbitrap). A "dilute and shoot" procedure, using acetonitrile with formic acid (1% v/v), was applied for the extraction of the target compounds. In this case, after this procedure, a clean-up step was necessary using a mixture of sorbents (PSA, GBC, C-18 and Z-Sep(+)). Parameters such as trueness, repeatability, intermediate precision, limits of detection and quantification were evaluated. Recoveries between 70 and 120% with relative standard deviation (RSD) values lower than 20% were obtained for 260 of the compounds. Limits of detection and quantification below 5 and 10 mu g kg(-1) were obtained, respectively. Nine samples of ginkgo biloba nutraceutical products were analyzed, finding pesticides in 8 out of 9 samples, such as hymexazol (10 mu g kg(-1)) and tebufenozide (55-459 mu g kg(-1)). In the case of mycotoxins, aflatoxin 81(5-54 mu g kg(-1)), aflatoxin B2 (4-300 mu g kg(-1)) and T-2 toxin (18-20 mu g kg(-1)) were found in 6 samples.
- 出版日期2015-1
